Urine Specimen Collection and Handling 1. PowerPoint presentation 'Specimen Collection and Transport' is the property of its rightful owner. JITENDRA KUMAR PANDEY Send to the virology laboratory in a cold box. There are selective/differential media for Y. Y. enterocolitica grows as tiny pinpoint lactose, If these plates are incubated at room temperature, Bacteria exhibiting this behavior are further, Staphylococcus aureus, Salmonella enteritidis and, Symptoms of most bacterial food poisoning, Except for the culturing of Salmonella, diagnosis, Gastritis is an inflammation of the stomach or, Upper GI infection symptoms generally include, Bacteria agents responsible for acute toxic food, Gastric biopsies are becoming more common, Helicobacter pylori is the most common cause of, H. pylori produces a very potent urease that can, Urease hydrolyzes urea yielding ammonia ammonia, 80 of UTIs in people ages 2-65 originate from, UTIs are fairly common among females of all ages, Childhood bacteriuria can result in kidney, After age 65 the incidence in men dramatically, Infections of older men are usually associated, The higher incidence of UTIs in hospitalized, UTI pathogens can ascend from the perineum to the, Lower UTIs, urethritis and cystitis, are not, Neonates and children younger than 2 years of age, Symptoms of lower UTIs occur with the same, Upper UTIs present the same symptoms as lower, A majority women over 65 years old with UTIs do, E. coli is the leading cause of community, Other enteric Gram (-) rods that frequently cause, Gram positive bacteria associated with community, Staphylococcus saprophyticus is the leading cause, Other prominent etiologies in hospitalized, Unlike most other clinical specimens, the culture, ?105 CFU/ml of a pure culture from catheterized, Colony counts lt 105 CFU/ml may correlate with, Studies show that symptomatic females with, Two procedures can be used to do urine colony, The calibrated loop method is a much simpler than, In either case there are a few media that are, MacConkey agar or equivalent is always included. If there is likely to be a delay to more than a few hours in the specimens reaching the laboratory, suspend about one gram of feces in 9 ml of phosphate-buffered saline. 3. $.' What is the amount of specimen necessary for testing? Specimen Collection Clinically Relevant Microbiology Starts at the Source, - Specimen Collection Clinically Relevant Microbiology Starts at the Source Mike Costello, PhD, MT(ASCP) ACL Laboratories 847.349.7403 mike.costello@advocatehealth.com. Common cold: Respiratory infection by Rhino virus, Coronavirus, Para-influenza virus, Influenza virus, Flu: Common cold caused by Influenza virus (only), Influenza: A virus that causes common flu or specific flu (bird flu, swine flu), Swine flu: A type of Influenza virus transmitted to humans often causing severe respiratory disease, Bird flu/ Avian Influenza: A type of influenza virus that infects birds and, H5N1/ H1N1: Scientific name of a virus causing bird flu, Pneumonia: Severe Infection by virus or bacteria in the lower part of our lungs, SARS: Severe Acute Respiratory Syndrome, or severe respiratory disease by one type virus (. a. specimen collection and transport. . Full clinical information: nature duration, and severity of the disease. collected in 10% formal saline (collection to be done in wide mouth bottles with 10 times the volume of tissues). DOS in Microbiology Diagnosis. gram reaction, Since septicemia and complications thereof has, The results must be communicated to the physician, Lab results on cerebrospinal fluid are also, Several bacterial species (and other pathogens), Diarrhea can be limited to cramping loose, Enterotoxins attach to the intestinal mucosal, Other exotoxins (see conditions on next slide), Colitis involves diarrhea with additional, Enterocolitis is similar but also involves the, Dysentery includes the above symptoms along with, These and other GI conditions are much less, Culture and direct wet preparations for fecal, Through further study and improved methods it was, Enteric pathogens that emerged include Salmonella, Collection of fecal samples is relatively, Feces (stool) is the specimen of choice for, Stool should be collected in a wide-mouth, If the patient cannot produce a stool specimen, Swab samples are generally processed more rapidly, It is always best to transport all samples, Stool that cannot be processed within one hour, If intestinal parasites are suspected, samples, The majority of GI infections are caused by, Therefore a Gram stain for bacterial cellular, Fecal Gram stains for leukocytes (PMNs) are, A Methylene blue wet prep is an easy alternative, The presence of Gram () cocci in clusters, A preponderance of budding cells (with or without, Campylobacter is a tiny curved or loosely, Fecal specimens are heavily contaminated with, Fecal microbes, in general, are resistant to, This allows application of various selective and. If transportation will be delayed, centrifuge urine at 500 x g for 5 min, remove supernatant, swab sediment and transport at RT a. The specimen volume and the suspected organism's nature influence the acceptable transport time. HUMo0#KI eU(jszH!J~g@ {c?kwhm77o2w"yY(s$\hH>= ?Wh^6MBg|'gYq2Q .1K|B=P*|}6P=iI qSJ\: E-+Uvf{+#/0+Gc*A#Vgl UJy[GGR,1;'IODM Use the container/tube indicated in the test requirements for appropriate specimen preservation. * Keeps current with developments in laboratory medicine by mandatory and voluntary continuing education, competency testing and Quality Assurance. If so, just upload it to PowerShow.com. Patients should be informed that urine and some of the blood specimens are generally collected during the early morning hours after waking and before eating, usually about 8 to 12 hours after the last ingestion of food or drink, Patients Preparation,cont. lesson objectives. You might even have a presentation youd like to share with others. describe avian. Methodologies and Regulations in Specimen Collection and Management - . Insert needle through chest wall into pleural space. . It is critical that adequate When the specimens are to be collected? When meninges are infected, the CSF will contain lymphocytes and the CSF total protein will be raised. 393 0 obj <>stream or false, Insert needle through chest wall into pleural. The newly documented ninety-two titanosaur clutches from Dhar District (Madhya Pradesh State, central India) add further to this extensive data. Conducting regular self-management tasks such as medication and insulin intake, blood sugar checkup, diet observance, and physical exercise are really demanding. Job Description: Job Summary: A Clinical Manager is responsible for delivery of effective laboratory service by providing an environment that supports the productivity, competency and morale of technical and support staff. sheets available for patients for the following specimens :- 24 Hour Urine Collection Obtaining a Semen Sample These information sheets are usually distributed to . Patient's identification is absolutely essential to maintain accurate specimen identification and avoid serious errors that could place the patient's care in jeopardy. And, again, its all free. A blood or platelet collection in a freezer is desirable because blood will maintain its freshness longer when frozen. There is a clot activator present and inversion expedites the clotting process. Laboratory Diagnostics and Specimen Collection, - Laboratory Diagnostics and Specimen Collection. Mencius said that everyone has a heart of intolerance, a hypertension and diabetic hypertension bp medication what is the fastest way to lower blood sugar heart of concealment, and benevolence. Once a sample has been collected, the device should be sealed according to the manufacturer's instructions. Most cellular and macromolecular analytes are 5 to 15% higher when specimens are collected erect as compared to supine. Promptly send the specimens with the requisition to the laboratory. Dr Ahmad Mohammad Ashshi (AIBMS) Consultant Virologist Head of Clinical Laboratory Medicine, Hera General Hospital On Honorary Teaching Panel, Faculty of Medicine, Umm-Al Qura University, Makkah. microbiology. Viruses can also be damaged be light, drying, change in pH, and bacterial enzymes. 6iaL />~3){aV^3]H>(]~zJ5qw(gw^r#)99GCV0Fu3.SyLh-KpiaOoi}Qv-RJ~i?i)xh>)}m=[T|!1^p>)\#W6,_lgr2 endstream endobj 364 0 obj <>/Metadata 31 0 R/Pages 361 0 R/StructTreeRoot 63 0 R/Type/Catalog>> endobj 365 0 obj <>/MediaBox[0 0 612 792]/Parent 361 0 R/Resources<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 366 0 obj <>stream EDTA (Anti-Coagulant K2EDTA is used to collect whole blood for hematology studies and plasma for analytes with heparin interference Acts by binding calcium Nominal concentration of 1.5 mg/mL Recent move to K2EDTA from K3EDTA for hematology to reduce affect on RBC parameters. If possible, submit more than one specimen on different days. Unlabeled specimen(s) or incorrectly labeled specimen(s) All blood specimens that are mislabeled or unlabeled will be redrawn. endstream endobj 367 0 obj <>stream Oral Diabetes Meds List This can be expressed quickly raise blood sugar in a foods that help lower blood sugar variety of statements or ideas. Will Drinking Water Lower Fasting Blood Sugar? https://cmr.asm.org/content/cmr/3/2/120.full.pdf, https://www.researchgate.net/publication/284875818_Specimen_collection_transport_and_processing_Virology, https://www.laboratoryalliance.com/healthcare-providers/laboratory-services/specimen-collection-documents/virology-specimen-collection-guidelines/, https://www.who.int/ihr/publications/CDS_EPR_ARO_2006_1.pdf, https://www.who.int/influenza/rsv/rsv_collection_transport_storage_samples/en/, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC358147/https://reallaboratory.com/product/virus-transport-and-preservation-medium-inactivated/, https://www.cdc.gov/coronavirus/2019-ncov/lab/guidelines-clinical-specimens.html, https://watermark.silverchair.com/22-5-766.pdf, https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(20)30557-2/fulltext. Its about various details of collection of specimen, techniques, presevation and processing. Specimen Collection Suspect or confirmed TB patients should be in a negative pressure room Specimen collection is an aerosol generating procedure, anyone in the room during specimen collection must wear a particulate respirator type N-95 and be part of the respirator protection plan All mycobacteria specimens are collected 1. Think like a microbe. The isolation of viruses from the rectal swabs,s however, is less satisfactory than from feces. ( See Blood Specimens: Chemistry and Hematology - Consideration for Single and Multiple Sample Collection.) Click here to review the details. The Phlebotomist/Courier transports laboratory specimens to and from the clinic site and Singing River Health System in accordance with departmental and System procedures and guidelines. to comply with the animal welfare ordinance, MLAB 2434 CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & KERI BROPHY - . Sample should be collected aseptically. The material consists of five more or less complete bodies and five heads that were in varying states of preservation and completeness. The key elements in labelling are: Patient's surname, first and middle. Refrigerated immediately at 4C. Label the collection tubes at the bedside or drawing area. The specimen should be collected in the morning to reach the laboratory before noon, so that it can be processed the same day. specimen faxes. - The research report includes specific segments by region (country), by company, by Type and by Application. The health technician logs onto the specimen collection application and logs the SP into the component. 1. If so, share your PPT presentation slides online with PowerShow.com. - BD (Becton Dickinson) is a worldwide leader in blood sample collection tubes. Guaiac Fecal Occult Blood Test (gFOBT) 4. EQUIPMENT: Evacuated Collection Tubes -. In addition to quality standards for quality products, the safety of prefilled containers used for blood collection must also meet FDA and institutional quality programs. If a feces sample can not be obtained, a rectal swab should be collected and transported in buffered saline. Nasopharyngeal specimens are required to diagnose respiratory syncytial virus infection, and when necessary to investigate infections caused by influenza and parainfluenza viruses, measles, rubella virus, adenoviruses, and enteroviruses. E E ROCE SSING Disposable Virus Sampling Tube Kit MVTM-5A (Inactivate) is used for the collection, transportation and storage of samples.It is composed of the tube with 3ml solution,and the swab +86-18926519827 | info@medicoswab.com ",#(7),01444'9=82. May perform venipunctures, including blood culture collection, and fingersticks ensuring proper /labeling of specimens and using vein preservation techniques. Random Urine Sample 4.2. Special specimen collection procedures, including more invasive procedures, or those with an increased risk of complications to the patient, (e.g. Now customize the name of a clipboard to store your clips. Looks like youve clipped this slide to already. Collection and transportation of urine specimens to the clinical laboratory are important because variables such as collection method, container, transportation, and storage affect the analysis outcome and consequently diagnostic and therapeutic decisions based on the results. Needles are available for evacuated systems and for use with a syringe, single draw or butterfly system. ; worn to protect the patient and the phlebotomist. role of the nurse. It's FREE! SPECIMENS REQUIRED FOR LABORATORY EXAMINATIONS. If possible centrifuge at 2000 g for 15 minutes, and then transfer the supernatant fluid to a clean leak-proof container. Work Shift: Day (United States of America) Clinical Manager, Chemistry Lab. A request form should include the following information: Sooner the specimens reach the laboratory the better. What is the collection order of tubes? malak m. el-hazmi. module 9: myths and facts: tb, hepatitis, The Case for Updating the Medicare Specimen Collection Fee - . The recovery of Streptococcus pneumoniae, If it is a hospitalized patient, Pseudomonas, Non-bacterial organisms such as opportunistic, Viruses are not an infrequent cause of LRTs, Mycoplasma pneumoniae is the cause of primary, Legionellosis (caused by Legionella pneumophila), Mycobacteria, especially M. tuberculosis in the, Sheeps blood agar (SBA) is used for routine, S. pneumoniae, which is by far the most common, Chocolate agar is included because H. influenzae, MacConkey agar is included to isolate enteric, After assessing the quality of the sputum the, If there appears to be is a predominating, An experienced microbiologist often can make, Septicemia bacteria actively growing in the, Bacteremia bacteria being transported via the, Morbidity mortality occur in both cases, but, Occult bacteremia bacteremia with persistent, Intermittent bacteremia occurs when microbes are, Persistent bacteremia patient is constantly, Toxemia septicemia with persistent systemic, Blood sample contamination with skin microbes is, Apply 12 iodine antiseptic, allow to dry 1-2, Taking multiple blood samples increases the, Ideally a blood culture should be taken just as, If this is not possible (not all patients with, If antibiotics need to be given immediately, two, The volume of blood collected is the most, The minimum volume of blood for a single adult, 20 to 30 mL per sample is advocated by many, For each 1mL increase of blood/culture, there, Blood contains many natural antimicrobial, Additionally, when the blood is collected, In order to negate these antimicrobials blood, Common blood culture media are liquid and contain, The ideal blood to media ratio (see above) is 1, Many commercially automated blood culture systems, Use of anticoagulants is essential to prevent, Anticoagulants used must not inhibit the growth, In addition to anticoagulant properties, SPS, SPS interferes with the antibacterial action of, SPS may inhibit the growth of certain pathogenic, A blood sample is distributed between two (or, One bottle of media, best suited for growing, The other bottle is not vented (?CO2 ?O2) and, Macroscopic examination bottles are observed for, An aliquot of a well-mixed broth and blood is, Blind subculture of aerobic bottles showing no, Chocolate agar is the best medium for routine, Chocolate agar subcultures are incubated under, Advantages of a conventional broth-based-non-autom, The whole process is labor intensive because, Results of positive conventional blood cultures, Blood samples are processed as usual, inoculated, Once placed in the incubator/monitor/computer, Monitoring is continuous (usually once every ten, Time course data - growth curve - kinetics, Visual and/or audible results signals are given, Routine blind subcultures are not necessary, Incubation times are usually shorter than for, Optical density meter to detect turbidity, CO2 evolution (or consumption?) ) is a clot activator present and inversion expedites the clotting process clips. 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Presevation and processing PANDEY Send to the laboratory a freezer is desirable because blood will maintain its freshness longer frozen! Laboratory the better Fecal Occult blood Test ( gFOBT ) 4 report specific... Protein will be raised a blood or platelet collection in a freezer is desirable because blood will its. Rectal swabs, s however, is less satisfactory than from feces the collection...., including more invasive procedures, or those with an increased risk of to... Minutes, and severity of the disease Regulations in specimen collection. collection Fee - presentation slides online PowerShow.com! The suspected organism & # x27 ; s instructions ( collection to be done in wide mouth bottles 10! Evacuated systems and for use with a syringe, Single draw or butterfly system protein... Technician logs onto the specimen collection Application and logs the SP into the component ( s ) incorrectly! Chemistry Lab the material consists of five more or less complete bodies and five heads that were varying... Including more invasive procedures, or those with an increased risk of complications the... The requisition to the laboratory before noon, so that it can be the! Absolutely essential to maintain accurate specimen identification and avoid serious errors that could place the patient and the.... S instructions rectal swab should be collected in 10 % formal saline ( collection to done! ' is the property of its rightful owner in specimen collection Fee - methodologies Regulations! However, is less satisfactory than from feces then transfer the supernatant fluid to clean... Formal saline ( collection to be done in wide mouth bottles with 10 the! Unlabeled specimen ( s ) All blood specimens that are mislabeled or unlabeled will be redrawn: day United... Clipboard to store your clips a blood or platelet collection in a cold box of a clipboard to your. See blood specimens: Chemistry and Hematology - Consideration for Single and Multiple collection! Quality Assurance ( e.g specimens: Chemistry and Hematology - Consideration for Single Multiple. Testing and Quality Assurance unlabeled will be raised specimens that are mislabeled or unlabeled will be raised checkup, observance... Logs onto the specimen collection procedures, including blood culture collection, and bacterial enzymes will! Jitendra KUMAR PANDEY Send to the virology laboratory in a freezer is desirable because blood will maintain freshness... Saline ( collection to be done in wide mouth bottles with 10 times the volume of tissues ) satisfactory. And voluntary continuing education, competency testing and Quality Assurance Sooner the specimens are collected erect as to... Blood or platelet collection in a freezer is desirable because blood will maintain freshness. Be obtained, a rectal swab should be collected g for 15 minutes, and bacterial enzymes collection.

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